monolayer | monolayer culture

confluent cell monolayer culture | confluent monolayer cell culture

monoplated nickel blank [ monoplated Ni blank | mono nickel plated blank | mono Ni plated blank ]

copper mono-plating process [ mono-ply copper-plating process | single-ply copper-plating process ]

self-assembled monolayer | SAM | self-assembling monolayer

Modifiers The following fourth-character subdivisions are for use with categories R83-R89: Code Title .0 Abnormal level of enzymes .1 Abnormal level of hormones .2 Abnormal level of other drugs, medicaments and biological substances .3 Abnormal level of substances chiefly nonmedicinal as to source .4 Abnormal immunological findings .5 Abnormal microbiological findings Positive culture findings .6 Abnormal cytological findings Abnormal Papanicolaou smear .7 Abnormal histological findings .8 Other abnormal findings Abnormal chromosomal findings .9 Unspecified abnormal finding | Positive culture findings | Abnormal Papanicolaou smear | Abno ...[+++]rmal chromosomal findings

single-layer perceptron | SLP | simple perceptron

mixed monolayer | multicomponent monolayer

150 μl cell culture supernatant in two different dilutions (1:10 and 1:1 000) shall be inoculated in duplicate onto 1-day-old monolayers and incubated at 15 °C for 24 hours;

specific monoclonal antibodies (namely for VHSV identification, MAb IP5B11 and for IHNV, Hyb 136-3 respectively) shall be diluted in 0,01 M PBST, pH 7,2 in the dilution recommended by the provider of the MAbs; 50 to 100 μl/well shall be added to the fixed monolayer and plates shall be incubated for one hour at 37 °C in a humid chamber;

The cell cultures to be used for inoculation with fish tissue material shall be young, normally 1 day old cell culture monolayers where possible; however, a range between 4 to 48 hours old may be accepted.

They are best seen by removing the agar overlay and staining the cell monolayer with crystal violet (0,5 % w/v) in 25 % v/v ethanol.