If the primary antibody response is assayed by counting plaque-forming cells, it is permissible to evaluate subgroups of animals on separate days, provided that: subgroup immunisation and sacrifice are timed so that PFCs are counted at the peak of the response; that subgroups contain an equal number of male and female offspring from all dose groups, including controls; and that subgroups are evaluated at approximately the same postnatal age.Exposure to the test chemical will continue until the day before collecting spleens for the PFC response or serum for the ELISA assay.

Animals that are sick or injured should not be considered fit for transport, except for slightly injured or sick animals whose transport would not cause additional suffering, or where the transport is under veterinary supervision for, or following, veterinary treatment.

(4) Regulation (EC) No 1774/2002 of the European Parliament and of the Council of 3 October 2002 laying down health rules concerning animal by-products not intended for human consumption(8), as amended by Commission Regulation (EC) No 808/2003(9), lays down animal and public health rules for the collection, transport, storage, handling, processing and use or disposal of animal by-products, including conditions for their use in animal feeding.

Transports of animals over 8 hours are conditional upon the authorisation of a route plan, the use of upgraded vehicles, and the respect of resting time at approved staging points for feeding, watering etc.

5.8. With the exception of vaccinations, treatments for parasites and any compulsory eradication schemes established by Member States, where an animal or group of animals receive more than two or a maximum of three courses of treatments with chemically-synthesised allopathic veterinary medicinal products or antibiotics within one year (or more than one course of treatment if their productive lifecycle is less than one year) the lifestock concerned, or produce derived from them, may not be sold as being products produced in accordance with this Regulation, and the livestock must undergo the conversion periods laid down in Section 2 of thi ...[+++]s Annex, subject to the agreement of the inspection authority or body.

- - - 1 - SERVICES FREE FROM AUTHORISATION REQUIREMENT Before After ------ ----- a) A few occasional services a) Almost all occasional services, namely : - closed door tours - services already free from - outward journey laden and return authorisation (see facing journey unladen column) - certain services - services carrying various groups of passengers on round trips - one-way services carrying groups of passengers between Member States - services organised for special occasions (conférences, etc.). b) - b) Shuttle services with accomodation (defined as at least 2 nights stay) ; these services may also stop at a maximum of 3 places en route passengers able to return on any subsequent service. Shuttle services with accomodation may carry a ...[+++] certain percentage of travel only passengers c) - c) Most special regular services (services carrying workers, school-children, military personnel + frontier urban transport), provided they are subject to a contract between organiser and carrier. d) Transport by non-profit making d) Transport by non-profit organisations subject to a variety of making organisations of national rules and provisions of their own members in their bilateral agreements own vehicules subject to certification - - - 2 - AUTHORISATION PROCEDURES Before After ------ ----- a) Applications for almost all services a) Applications for regular and to be examined except in light of shuttle services without effects on existing markets (all accomodation to be allowed transport modes) and taking account unless proposed service of whether operators already in market would threaten existing could organise such a service regular coach services or themselves comparable rail service or is aimed exclusively at the most lucrative existing services. b) Countries where passengers are b) Countries where passengers picked up or set down and transit are picked up or set down countries may oppose a request for may oppose a request for authorisation authorisation c) No time-limit ...